Irvine Chemistry Lab
X-Lyz™ Protein Quantitation Assay

Total Protein Quantitation
 



Features

  • No need to generate standard curve each time
  • Wide linear range up to 10,000µg/mL
  • Quick and simple procedure
  • Low protein variations
  • Simple procedure to remove interferents
  • Compatible with reducing agents
 



Contents

  1. X-Lyz™ Protein Assay Reagent A
  2. X-Lyz™ Protein Assay Reagent B
  3. TCA Solution (remove interferent)
  4. BSA Standard
  5. User Instructions
 
Please see the MSDS for safely handling.
Contact us for questions, testing services
and new applications.
 
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Product Description

The  X-Lyz™ protein assay is a simple
procedure to measure the total protein
concentration in solution and solid. The
proprietary assay involves a reaction
with primary amines of proteins to form
a chromophore. It exhibits strong UV
absorbance at 350-405nm linearly
proportional to concentrations over a
broad range of 10-10,000µg/mL.  The
rapid color development is complete
after 15min incubation.   A study shows
the calibration is stable over a year; it
does not require to re-generate the
standard curve each time.

The X-Lyz™ reagent is compatible with
commonly used substances in
chemistry and biology labs, such as
reducing agents, detergents, alcohols,
carbohydrates, and inorganic salts. The
method is suitable for assays in
microplates and test tube (cuvettes).  
More details may be found in the
instruction
.
 
 
 
Catalog #
User
Instructions
# of Assays
Microplate
# of Assays
Test Tube
Price
 
IC286094-1
Download PDF
3200
480
$70
IC286094-2
Download PDF
6400
960
$100

Introductory Video
 
 
 
 



Broad Linear Range

The X-Lyz™ assay has a broad linear range up to 10,000
µg/mL (
Figure 1), while other methods, such as BCA and
Bradford assays, are lower than 2000µg/mL.


Figure 1: Standard Curve of BSA. The analysis was conducted with
sample size 25 µL and WR/Sample 10/1 in 96 well microplate.
 


Accurate Estimation

The estimation of the protein samples has an excellent
agreement with the true values as shown in
Table 1.



Table 1: Estimates of BSA Samples from the Standard Curve in
Figure 1.
 
True Values
(µg/mL)
Average
Absorbance
Estimates
(µg/mL)
Deviations
200
0.049
215.1
7.5%
300
0.074
301.2
0.4%
600
0.160
597.4
-0.4%
900
0.260
941.9
4.7%
1900
0.562
1982.1
4.3%
5000
1.406
4889.2
-2.2%
9000
2.600
9001.8
0.0%
 


Long Calibration Stability

The kit has shown a highly repeatable performance and
does not require to generate standard curve each time.  
In a stability study, twelve different assays were
conducted over a year using the same standard curve.
The average absolute deviations of the estimates were
shown to be smaller than 4%, while the maximum was
within 10% (
Table 2).

Table 2: Deviations of Estimates of Protein Samples. The analysis
was conducted with sample size 75 µL and WR/Sample 1/1 in 96
well microplate.
True Values: The true concentrations of BSA samples in µg/mL.
Average Absorbance: Average absorbance in five replicates.
Estimates: Estimates of sample concentrations.
Deviations: Percentage deviations of the estimates over the true values.
 



Low Level of Protein Variation

The X-Lyz™ reagent has shown a less variation among
different proteins compared to other assays (
Table 3).




Table 3: Comparison of Protein Variations in Different Methods
 
Concentration
Average
Minimum
Maximum
100µg/mL
3.8%
0.9%
7.0%
200µg/mL
3.1%
0.2%
9.0%
300µg/mL
2.7%
0.3%
6.9%
600µg/mL
2.9%
0.7%
6.1%
900µg/mL
1.7%
0.3%
3.3%
1900µg/mL
2.7%
0.6%
5.4%
Assays
X-Lyz™
BCA
Pierce 660
Bradford
Coefficient of
Variation
8.7%
15%
37%
44%
 
Concentration: The true concentrations of the tested proteins samples.
Average: The average deviations of the estimates in twelve assays.
Minimum: The minimum deviations of the estimates in twelve assays.
Maximum: The maximum deviations of the estimates in twelve assays.